Training in Vaccinology -Cytometry by time‐of‐flight
|Date & Time||September 19-20, 2019|
|Address||18 route du Panorama, 92265, Fontenay-aux-Roses, France|
|Location||CEA, Fonternay-aux Roses, France|
The course will comprise theory and practice of mass-cytometry applied to assessing the immune response in human and animal models. Cytometry by time‐of‐flight (CyTOF), or mass cytometry, allows the simultaneous detection of multiple phenotypic markers at the single cell level by making use of antibodies and specific probes conjugated with pure heavy metal isotopes. Labelled cells are then detected by atomic mass spectrometry. Whereas most conventional flow cytometry can be used to measure fluorescence in a maximum of 18 channels, with considerable spectral overlap requiring ad hoc mathematical processing, CyTOF technology makes it possible to measure more than 50 different parameters (in theory 130), with minimal spectral overlap. The course will describe the technology in details, and will present methods for characterizing surface markers and intracellular markers (cytokines and degranulation markers), allowing the characterization of activation, differentiation and functionality of lymphoid and myeloid cells. The training will be particular focused on new methods for unsupervised analysis of high dimensional data generated by flow and mass cytometry.
INTERESTED IN THIS COURSE?
The course is offered free of charge. However, selected applicants will have to cover their own travel and visa expenses and accommodation. Participants will be selected by a course selection panel.
For more information about the training modules offered by TRANSVAC, the eligibility criteria and the application process, please visit here.
TRANSVAC2 TRAINING MODULES
The training module is part of a series of 14 training modules dedicated to vaccinology set up by the TRANSVAC2 consortium at leading European centres. The modules can be combined to create customised international courses on vaccine R&D. Two rounds of customised training courses are planned. Participants can select topics as needed in their field of vaccine development, and the timelines of the various modules will be harmonised in a way that allows a logical continuation from one topic to the other.